The understanding of the wine process production only began to be unveiled in the second half of the 19th century, when microorganisms – yeasts – were discovered, transforming the sugar from the grape into alcohol. Later it was discovered that wine transforms itself into vinegar because of the acetic bacteria in the presence of air and that this bacteria ceased its activity when the wine had above 14 percent of alcohol by volume. It was later discovered that, in the absence of air, some lactic acid bacteria transformed the malic acid of the grapes into lactic acid, producing carbon dioxide, which makes the majority of red wines softer and better preserved.